EFFECTS OF CRYOPRESERVATION ON SPERM DNA FRAGMENTATION AS ASSESSED BY SPERM CHROMATIN DISPERSION TEST IN NORMOZOOSPERMIC EJACULATED SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation.
Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation in semen. The SCD test is based on the principle that sperm with fragmented DNA fail to produce the characteristic halo of dispersed DNA loops that is observed in sperm with non-fragmented DNA, foll...
متن کاملSperm nuclear DNA fragmentation and chromatin structure in one-day-old ejaculated sperm
OBJECTIVE To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours'incubation at room temperature. METHODS Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the...
متن کاملEffect of chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men
Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin co...
متن کاملEnvironmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).
Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...
متن کاملEffects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm
Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmen...
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ژورنال
عنوان ژورنال: Journal of Evidence Based Medicine and Healthcare
سال: 2019
ISSN: 2349-2562,2349-2570
DOI: 10.18410/jebmh/2019/19